| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206819 |
| Chromosome: | chromosome 15 |
| Location: | 1085915 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g638303 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCCTCGCCCAAGGCCCGCAGCGCCTGC |
| Internal bar code: | CGAGGCGGCCTCAGCTCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 291 |
| LEAP-Seq percent confirming: | 97.3026 |
| LEAP-Seq n confirming: | 4004 |
| LEAP-Seq n nonconfirming: | 111 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAACACTAGGCCGGACAC |
| Suggested primer 2: | GTGTGGGTTCATGCTAGGGT |