| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206833 |
| Chromosome: | chromosome 17 |
| Location: | 6214786 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g742050 | (1 of 18) IPR011051 - RmlC-like cupin domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAGAAGAAGCGAAGACGGACGGTCCAA |
| Internal bar code: | TAGGCCGCTATACTTACCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 933 |
| LEAP-Seq percent confirming: | 99.0751 |
| LEAP-Seq n confirming: | 3428 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGAGCACGTCGATAAGC |
| Suggested primer 2: | AAAGCCTGGTACGTGTGGAC |