Insertion junction: LMJ.RY0402.206848_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.206848
Chromosome:chromosome 16
Location:1400077
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g652150 FBP4 (1 of 1) K01103 - 6-phosphofructo-2-kinase / fructose-2,6-biphosphatase 3 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCCGTGTTCGCGCTTTTCGGCACATGCC
Internal bar code:CCGCGCCTGCTGGGCTGTTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:742
LEAP-Seq percent confirming:99.6834
LEAP-Seq n confirming:2204
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CACGGACTCACATGGTAACG
Suggested primer 2:CCATGTGGAATAGGAATGGG