| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.206860 |
| Chromosome: | chromosome 16 |
| Location: | 5655764 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679350 | (1 of 3) IPR003131//IPR011333 - Potassium channel tetramerisation-type BTB domain // POZ domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAATGTTTCCCGTAGTGTTGTGTAATGC |
| Internal bar code: | TTTTGTTGCTAAACTGTCTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 515 |
| LEAP-Seq percent confirming: | 99.8053 |
| LEAP-Seq n confirming: | 1025 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCTGCTGAGATGGTTGT |
| Suggested primer 2: | TGTGGGTGTAGGGACGTGTA |