| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206860 |
| Chromosome: | chromosome 16 |
| Location: | 5655775 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679350 | (1 of 3) IPR003131//IPR011333 - Potassium channel tetramerisation-type BTB domain // POZ domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCTCGTCTCGCTCTCATCGCTCGCGGTG |
| Internal bar code: | AAGACGCTTAGCGACGCCGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 226 |
| LEAP-Seq percent confirming: | 99.4042 |
| LEAP-Seq n confirming: | 1001 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCTGCTGAGATGGTTGT |
| Suggested primer 2: | TGTGGGTGTAGGGACGTGTA |