| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206876 |
| Chromosome: | chromosome 8 |
| Location: | 495242 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358850 | FAP386 | Flagellar Associated Protein 386 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGGGTTAATGCCGGCGGGGAATGCATGA |
| Internal bar code: | CGGCAGAGCCAATTCAAGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 107 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 245 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGACTTTGCCCACAGTAAT |
| Suggested primer 2: | GGGTGAATGAGGAAGCAAAA |