| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206889 |
| Chromosome: | chromosome 3 |
| Location: | 2811615 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162300 | SDR7 | Short-chain dehydrogenase/reductase; (1 of 13) 1.1.1.300 - NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACGCCGGCAAACCCGGTAAACGTTATT |
| Internal bar code: | GGGCGGTAGGGGGCTCGTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 739 |
| LEAP-Seq percent confirming: | 99.7535 |
| LEAP-Seq n confirming: | 3237 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCCCAAAACACTGACAC |
| Suggested primer 2: | TCAAGCTTGCAACCATCAAG |