| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.206901 |
| Chromosome: | chromosome 16 |
| Location: | 4556353 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGGATACTAAACGTGTGCCTAGTCTGC |
| Internal bar code: | TCACACTCTTACTCTTGGTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 99.5594 |
| LEAP-Seq n confirming: | 8134 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCATCGTCATTCTGTCTT |
| Suggested primer 2: | CACAAGACCGTTTCGCTGTA |