Insertion junction: LMJ.RY0402.206902_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.206902
Chromosome:chromosome 6
Location:3199115
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g275950 FAP395 Flagellar Associated Protein 395 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTAGCCGCTGCTTGAGCTGAGGGAAGGT
Internal bar code:GGCAACAAGGCAGAGCGGGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:295
LEAP-Seq percent confirming:99.7235
LEAP-Seq n confirming:4689
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CTACTGCATTGGGTTCGGTT
Suggested primer 2:AGCAAGAGGAGGAGGAGGAG