| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.206918 |
| Chromosome: | chromosome 3 |
| Location: | 1100209 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g148800 | (1 of 1) 2.1.1.4 - Acetylserotonin O-methyltransferase / Hydroxyindole O-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCTAAGGATTCCCGCACGCGCCGAGGTT |
| Internal bar code: | CTGCCGATGCTTCTCGTATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 820 |
| LEAP-Seq percent confirming: | 97.9667 |
| LEAP-Seq n confirming: | 1060 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGATTATTGTGCAACAC |
| Suggested primer 2: | CAATGACAGCTCGTTTGCAT |