| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206929 |
| Chromosome: | chromosome 16 |
| Location: | 2273682 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659000 | ATG13 | (1 of 1) K08331 - autophagy-related protein 13 (ATG13); AuTophaGy related | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCCCGTGCCTCCCACGTGCCTCCCACG |
| Internal bar code: | ATCAGAAGCCCCGAGGGTCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 335 |
| LEAP-Seq percent confirming: | 99.6602 |
| LEAP-Seq n confirming: | 2933 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACCTACCACACACACAC |
| Suggested primer 2: | GTGTTCTGTGTGTGTGCGTG |