| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206960 |
| Chromosome: | chromosome 2 |
| Location: | 4128004 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097900 | AST3 | (1 of 1) K00811 - aspartate aminotransferase, chloroplastic (ASP5); Aspartate aminotransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTGAACCAATCCCGTGTGTGTGTGTGTG |
| Internal bar code: | CGGCTCCGTGCTGGAAACGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4 |
| LEAP-Seq percent confirming: | 29.0592 |
| LEAP-Seq n confirming: | 383 |
| LEAP-Seq n nonconfirming: | 935 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGTTTGAGGTGTGAGGT |
| Suggested primer 2: | TCATAGCACCCCTTAGCACC |