| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206966 |
| Chromosome: | chromosome_2 |
| Location: | 1250431 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g082550 | ZEP,ZEP1 | Zeaxanthin epoxidase | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGCCCTTCTGCACACATTCGTACATTCTG |
| Internal bar code: | GTCAGAACCCTGAGGACTGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 221 |
| LEAP-Seq percent confirming: | 99.8656 |
| LEAP-Seq n confirming: | 743 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTAATTGCGTGTTGGTGG |
| Suggested primer 2: | TCAAGGTGAAGATGCAGCAC |