| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.206968 |
| Chromosome: | chromosome 10 |
| Location: | 5230605 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457050 | (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGGCGAGACAGCGCGCGATGTGCGCCT |
| Internal bar code: | GTCTCGACTGGTGTCATTTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 310 |
| LEAP-Seq percent confirming: | 98.0583 |
| LEAP-Seq n confirming: | 202 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCAAAGTGGGTAAGCCAA |
| Suggested primer 2: | GCGGTTTGCAATGTTATCCT |