Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.206968 |
Chromosome: | chromosome 10 |
Location: | 5230612 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g457050 | (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGAACCTAATTGTCTCTATAAGCCTCAA |
Internal bar code: | CTAAGATTGATTTTAAAAGGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 779 |
LEAP-Seq percent confirming: | 99.6013 |
LEAP-Seq n confirming: | 2498 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCCAAAGTGGGTAAGCCAA |
Suggested primer 2: | GCGGTTTGCAATGTTATCCT |