| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.207001 |
| Chromosome: | chromosome 7 |
| Location: | 3437335 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335300 | KAS2 | (1 of 1) 2.3.1.179 - Beta-ketoacyl-[acyl-carrier-protein] synthase II / KASII; 3-ketoacyl-ACP-synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAAGCTTCGATACAGCTGAGCATCTTGG |
| Internal bar code: | GAGCGGGTCGAGGAACGCAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 958 |
| LEAP-Seq percent confirming: | 99.6911 |
| LEAP-Seq n confirming: | 6455 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACTGTGCGTAATGCTGGC |
| Suggested primer 2: | AGACATGTTGGTCCGTGTGA |