Insertion junction: LMJ.RY0402.207026_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.207026
Chromosome:chromosome 13
Location:3938471
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g590700 (1 of 4) PTHR16255//PTHR16255:SF4 - UNCHARACTERIZED // SPORULATION PROTEIN RMD8 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGTTCAACACTCCGTGGTACGTCCCTCC
Internal bar code:AGGGATCCGTTCCCAAAATGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:226
LEAP-Seq percent confirming:99.6604
LEAP-Seq n confirming:4108
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGGCCTTTACAACTCTGC
Suggested primer 2:GTTTCGCTGGGTTTGGTTTA