| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.207049 |
| Chromosome: | chromosome 2 |
| Location: | 3183101 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095068 | (1 of 1) PTHR15535//PTHR15535:SF18 - TRANSMEMBRANE PROTEIN 2-RELATED // COMMUNICATION MUTANT PROTEIN F-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCCCGCCCCGCCCGCTCGCACGTGTGC |
| Internal bar code: | TACTCGCACTGCTCTTGCTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 99.8243 |
| LEAP-Seq n confirming: | 11933 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATGGAACTGAGGAGGGA |
| Suggested primer 2: | GGAGAAAGGTGGGAGAAAGG |