Insertion junction: LMJ.RY0402.207076_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.207076
Chromosome:chromosome 11
Location:706070
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467626 (1 of 8) 2.7.11.1//2.7.12.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Dual-specificity kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTGGCCAAGATTGTGATGCAAGGATGGAC
Internal bar code:TTAGTATCTGCGCACCAGGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:762
LEAP-Seq percent confirming:98.7153
LEAP-Seq n confirming:2920
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AACTTTTGTTGATGCCGTCC
Suggested primer 2:GGACAATTGGTTCATGGGAC