| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.207105 |
| Chromosome: | chromosome 3 |
| Location: | 2115796 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157000 | ELG1 | Exostosin-like glycosyltransferase 1; (1 of 1) PF12661 - Human growth factor-like EGF (hEGF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCTCGCTCATGGGCTCTAAAGCACATGC |
| Internal bar code: | GACGATGGTCGTATGGTTAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1006 |
| LEAP-Seq percent confirming: | 96.8158 |
| LEAP-Seq n confirming: | 3527 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGCGTGTGAGTCAGGTC |
| Suggested primer 2: | TTGCAGAGATCACAACGTCC |