Insertion junction: LMJ.RY0402.207109_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.207109
Chromosome:chromosome 2
Location:580442
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g077650 (1 of 2) IPR000340//IPR000387//IPR029021 - Dual specificity phosphatase, catalytic domain // Tyrosine specific protein phosphatases domain // Protein-tyrosine phosphatase-like 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCCGCGGTCGCGGTTAGTCCCGCAAAG
Internal bar code:CAACCAGACACGCAATCGGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:609
LEAP-Seq percent confirming:99.4841
LEAP-Seq n confirming:5978
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCACTTATGGTACAAGCG
Suggested primer 2:TTCTTACCAGGGGCTCAAGA