| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.207119 |
| Chromosome: | chromosome 12 |
| Location: | 8213326 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550550 | CYG48 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTACGCCATACGTCACAACACCGCAGCGC |
| Internal bar code: | CGGATTTAGAAAGCACGGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 640 |
| LEAP-Seq percent confirming: | 94.8299 |
| LEAP-Seq n confirming: | 4879 |
| LEAP-Seq n nonconfirming: | 266 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGGATAGCCACAGGCTC |
| Suggested primer 2: | CTTCTTCAGCGGGTTCTCAG |