| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.207129 |
| Chromosome: | chromosome 2 |
| Location: | 3173372 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095067 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCACAAGGGTCGCAGCCCGCAAGCAGCG |
| Internal bar code: | GCCGCTGAGCCTGCTAGGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 857 |
| LEAP-Seq percent confirming: | 93.348 |
| LEAP-Seq n confirming: | 8939 |
| LEAP-Seq n nonconfirming: | 637 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTTGGACTGCCGCTACT |
| Suggested primer 2: | CAGTCGCGTCAGGACTCATA |