Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.207224 |
Chromosome: | chromosome 2 |
Location: | 4958530 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g103900 | MAPKKK11 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK; Mitogen-Activated Protein Kinase Kinase Kinase | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCTCGGCTAGCTGTGATATGTTGTCCTG |
Internal bar code: | GGTCTTGAAGTAAGCGGGGCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 528 |
LEAP-Seq percent confirming: | 99.8433 |
LEAP-Seq n confirming: | 1274 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCACGATAGACGACAGCAGA |
Suggested primer 2: | TGTCTCTGTCAACCGACTGC |