| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.207234 |
| Chromosome: | chromosome_17 |
| Location: | 3962547 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g728700 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCCGCGCCCATCGCCCTCCGTGAGCTCTG |
| Internal bar code: | CGAAGCGCTCGGCTGCTCAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 874 |
| LEAP-Seq percent confirming: | 99.866 |
| LEAP-Seq n confirming: | 3725 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCGTCCGAAAGCTACTC |
| Suggested primer 2: | CACTTGCGACTTCCTTGTCA |