Insertion junction: LMJ.RY0402.207239_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.207239
Chromosome:chromosome_17
Location:3202085
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre17.g722150 PKS3 Type III polyketide synthase antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):AATTGGTGTTGTGCCAAGCCCCTACAACGC
Internal bar code:CTCAAAGACGTCACACTCGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:777
LEAP-Seq percent confirming:99.6752
LEAP-Seq n confirming:3990
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CGGATGAGCTGTGTAGACGA
Suggested primer 2:CTGCAGCTCACAAACGACAT