| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.207245 |
| Chromosome: | chromosome 16 |
| Location: | 777388 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690750 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAGGGCGCTGTGTGGCGCCTTGCCCAAC |
| Internal bar code: | AGAGGTTCTTGGGGATTTTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 701 |
| LEAP-Seq percent confirming: | 99.6897 |
| LEAP-Seq n confirming: | 3855 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGTGCTGGAGAGCAAC |
| Suggested primer 2: | CAGAGCACATGAAAAGCCAA |