| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.207245 |
| Chromosome: | chromosome 16 |
| Location: | 777391 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690750 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGATTTTAGCCGGCCGCCGGGGACCGC |
| Internal bar code: | CCGACCAGGGCTGCTCGAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 618 |
| LEAP-Seq percent confirming: | 99.5366 |
| LEAP-Seq n confirming: | 1074 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGTGCTGGAGAGCAAC |
| Suggested primer 2: | CAGAGCACATGAAAAGCCAA |