Insertion junction: LMJ.RY0402.207279_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.207279
Chromosome:chromosome 7
Location:4680397
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g344702 (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAACCATGCGACTCGCAATCCGCTGTTT
Internal bar code:TGTCGCTCACTTTCACCAATTC

Confirmation - LEAP-Seq

LEAP-Seq distance:187
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CAACACAAAGGCACTCTCCA
Suggested primer 2:CTAGGACTGACCCAAGCAGC