Insertion junction: LMJ.RY0402.207288_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.207288
Chromosome:chromosome 2
Location:7015672
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g147700 (1 of 1) PTHR11271//PTHR11271:SF33 - CHLOROHYDROLASE FAMILY PROTEINS // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGCTCTGTATTTACATGCCCGTGCACTC
Internal bar code:AGGTGGGGACGGATTAGATGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:822
LEAP-Seq percent confirming:99.8645
LEAP-Seq n confirming:2211
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCTAATCTAGGCTCATCG
Suggested primer 2:TGTGACTGCTTGGACTCGAC