| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.207305 |
| Chromosome: | chromosome 5 |
| Location: | 1436137 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244050 | Chlamydomonas specific protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGTGACCAAGTCTGCTGTCGCTGTAGGC |
| Internal bar code: | CAGTAACCATGAGCGCGCATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 610 |
| LEAP-Seq percent confirming: | 99.7331 |
| LEAP-Seq n confirming: | 5978 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGTACGACCTGGACAAT |
| Suggested primer 2: | TTTTGATTGCGTGAGAGCAC |