| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.207325 |
| Chromosome: | chromosome 12 |
| Location: | 2713180 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503650 | SMM40 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) PTHR10108//PTHR10108:SF894 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATACACCAGGCAGAGGATATTCAAGGGC |
| Internal bar code: | CCCGCGCTTGTCATTGACATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 546 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCCTTGCTCCCTCTCCT |
| Suggested primer 2: | GAATGGCTGACTTGCACTGA |