Insertion junction: LMJ.RY0402.207326_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.207326
Chromosome:chromosome 12
Location:5873063
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g533800 CYN22 Cyclophilin 22; (1 of 1) K09567 - peptidyl-prolyl isomerase H (cyclophilin H) (PPIH, CYPH) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCGTCAGCGAACTACCAGCCCGTATCA
Internal bar code:ACGGAAGGATTCGAGTGTCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:854
LEAP-Seq percent confirming:99.0993
LEAP-Seq n confirming:3851
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GACGTGGCACACAAAATGAC
Suggested primer 2:TTGAAAGTGTCAGCACGGAG