| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.207340 |
| Chromosome: | chromosome 3 |
| Location: | 5399000 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184800 | (1 of 1) 3.6.1.42//3.6.1.6 - Guanosine-diphosphatase / GDPase // Nucleoside diphosphate phosphatase / Nucleoside-diphosphatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAAAGGCCGCCAGGTTCCACTGTCAAGT |
| Internal bar code: | TGTTTGAGCGCAAACTTGTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 97.6459 |
| LEAP-Seq n confirming: | 954 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGAGTCTTTCACTTCTCCG |
| Suggested primer 2: | GGCTAGGTTTTGGACGCAT |