| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.207343 |
| Chromosome: | chromosome 6 |
| Location: | 3752169 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278154 | (1 of 1) PTHR31560:SF0 - UPF0652 PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCATCAGCATGTACATCAGCTTGCCGTA |
| Internal bar code: | AAGCGGGCGTCGATTCGCGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 824 |
| LEAP-Seq percent confirming: | 99.5793 |
| LEAP-Seq n confirming: | 6154 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTTGTCGGACAGGGAGT |
| Suggested primer 2: | CCGATCATACAGCTTCAGCA |