Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.207348 |
Chromosome: | chromosome_7 |
Location: | 4487271 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g343400 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCCATGCCGAAGTCGACAGGCCGGCGCAT |
Internal bar code: | GGTACCCTGGGAGCGTGGGGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 423 |
LEAP-Seq percent confirming: | 99.9206 |
LEAP-Seq n confirming: | 5032 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCGGGGTGCCTTTACTACA |
Suggested primer 2: | CGGGTTTGGAGAGTGTTTGT |