| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.207359 |
| Chromosome: | chromosome 3 |
| Location: | 5835561 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189200 | (1 of 1) PTHR13192//PTHR13192:SF3 - MY011 PROTEIN // METHYLMALONIC ACIDURIA AND HOMOCYSTINURIA TYPE D PROTEIN, MITOCHONDRIAL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTTCACAGTTGAGCTCTGGGGCTTCTT |
| Internal bar code: | AATGGGGGGATGCGGTAGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 562 |
| LEAP-Seq percent confirming: | 99.9144 |
| LEAP-Seq n confirming: | 2334 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGATCCACACGGACCTC |
| Suggested primer 2: | ACTCCACGCCAATGGTAGAC |