| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.207360 |
| Chromosome: | chromosome_7 |
| Location: | 2340765 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g328075 | sense | 3'UTR|outside_mRNA | ||
| Cre07.g328150 | PDI4 | Protein disulfide isomerase | antisense | 3'UTR|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTGGAGGTCAGGGTGCTGTGAGCGCTGCA |
| Internal bar code: | ATGGTCCCTGCTGTCTGTCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 95.5989 |
| LEAP-Seq n confirming: | 4605 |
| LEAP-Seq n nonconfirming: | 212 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCTAAGCACCACAGTGA |
| Suggested primer 2: | CCTCAACGAGGAGCTGTAGG |