| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.207368 |
| Chromosome: | chromosome 9 |
| Location: | 4213584 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394288 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGCAGAATGCCACAAGCTCTCCGGCGT |
| Internal bar code: | GGCTAGCAGGTCGCCCTTCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 97.8617 |
| LEAP-Seq n confirming: | 3707 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAGCACACCCAGAATGCC |
| Suggested primer 2: | GTTCAAGCCAAGCCAAGAAG |