| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.207375 |
| Chromosome: | chromosome_2 |
| Location: | 5220352 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g105950 | FAP174 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGATGTAGCTGTCGAGGGGGGGGGGGCAA |
| Internal bar code: | ACGCGAAGGTGAGGACGACATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 666 |
| LEAP-Seq percent confirming: | 99.0245 |
| LEAP-Seq n confirming: | 3756 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAACATGCCCTTTTTGGTG |
| Suggested primer 2: | GTTTGAGCCTTGGAGCTCTG |