| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.207375 |
| Chromosome: | chromosome 16 |
| Location: | 3183657 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g666250 | TPT26,TPT27 | (1 of 2) K15281 - solute carrier family 35 (SLC35D); UDP-N-acetylglucosamine transporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTACACATGGCCCAGTATCTTCTGCGCT |
| Internal bar code: | TTCGGATTTCTGTAGTCTATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 90 |
| LEAP-Seq percent confirming: | 84.083 |
| LEAP-Seq n confirming: | 243 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTTGCTTGTAACAGGGC |
| Suggested primer 2: | CCTGCAGACGACAAACAAAA |