| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.207378 |
| Chromosome: | chromosome 1 |
| Location: | 4325891 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029300 | TPI1,TPIC1,TPIC | (1 of 1) 5.3.1.1 - Triose-phosphate isomerase / Triosephosphate mutase; Triose phosphate isomerase, chloroplast precursor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCATGGTGCAAAATTGGAGTGCACTGCAC |
| Internal bar code: | TCCAGGAAAAAGCCAAGTTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 257 |
| LEAP-Seq percent confirming: | 99.5434 |
| LEAP-Seq n confirming: | 218 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTGCAAGGACCTCTCGAA |
| Suggested primer 2: | TTGAGACACCTCACGCTGAC |