| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.207414 |
| Chromosome: | chromosome 10 |
| Location: | 5524754 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459350 | OPR49,RAP5 | OctotricoPeptide Repeat protein 49; (1 of 2) IPR000104//IPR013584 - Antifreeze protein, type I // RAP domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTGCTGGGCCGGCGGCGGCGGGGCCGCC |
| Internal bar code: | TTGTTCATTCAAAACGGGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGAGGAATGCATGTGAG |
| Suggested primer 2: | CCGTACCTTGCTTGTCCATT |