Insertion junction: LMJ.RY0402.207415_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.207415
Chromosome:chromosome 7
Location:360694
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g314700 (1 of 1) PF14675 - FANCI solenoid 1 (FANCI_S1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACCCCACATCTGTGTCGTCTGTCGCACC
Internal bar code:CTACCAGTACTAAAGACTTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:582
LEAP-Seq percent confirming:99.4937
LEAP-Seq n confirming:393
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CAGAGGAGATCCTGAGGCTG
Suggested primer 2:GTGTCCACACACTCTCCCCT