| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.207431 |
| Chromosome: | chromosome 16 |
| Location: | 3838257 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689851 | 3'UTR_intron|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATTACTTCCTCGCTTGGGTGCCTATGCG |
| Internal bar code: | TAATCGCATAACGTTTGAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1921 |
| LEAP-Seq percent confirming: | 93.985 |
| LEAP-Seq n confirming: | 125 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTCAGAGTCGTTGAGGT |
| Suggested primer 2: | TGTTTATGACAGCAGCAGGC |