| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.207432 |
| Chromosome: | chromosome 13 |
| Location: | 1890221 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575800 | IGS1 | Indole-3-glycerol-phosphate synthase; (1 of 2) 4.1.1.48 - Indole-3-glycerol-phosphate synthase / Indoleglycerol phosphate synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGGCGTGGGGTGGGTTGTGCAGCAGGG |
| Internal bar code: | CCGCAGGTCTATTTAATGAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 321 |
| LEAP-Seq percent confirming: | 99.7712 |
| LEAP-Seq n confirming: | 436 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGATTGTGTGTGGCAGTA |
| Suggested primer 2: | GCTACAAACTCTGCCAAGCC |