| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.207432 |
| Chromosome: | chromosome 13 |
| Location: | 1937939 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576250 | (1 of 1) K15202 - general transcription factor 3C polypeptide 5 (transcription factor C subunit 1) (GTF3C5, TFC1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTACAGCTTCGCTCCCGAGGGAGCCACGC |
| Internal bar code: | ACTTGATGAATGCATGGCAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 583 |
| LEAP-Seq percent confirming: | 99.3402 |
| LEAP-Seq n confirming: | 2409 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAACCAAGCCGGAACTAT |
| Suggested primer 2: | CCCTCATGGAGCAGATGAAT |