| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.207481 |
| Chromosome: | chromosome 11 |
| Location: | 1635901 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467768 | (1 of 1) K10277 - lysine-specific demethylase 8 [EC:1.14.11.27] (KDM8, JMJD5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACTAGAACGTTCTTGCAATCTCGCGATT |
| Internal bar code: | ACTGTAGATGGTATCTGCGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 37 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCTTTGGGTGACCAAGT |
| Suggested primer 2: | GTCTGCAAAGACATGAGCCA |