Insertion junction: LMJ.RY0402.207499_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.207499
Chromosome:chromosome 13
Location:3736895
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g589400 PRL6 (1 of 14) PTHR10334 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED; Predicted extracellular protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAACTCCTCAACACCTCTGCGCACTCCTG
Internal bar code:TACCGGCTATTATCACAGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:353
LEAP-Seq percent confirming:99.7925
LEAP-Seq n confirming:10582
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TACTGTACTGGGAAACGGGC
Suggested primer 2:GAGGCCAACCTGTGTTTCAT