Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.207500 |
Chromosome: | chromosome 6 |
Location: | 2818337 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g271450 | (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCCACACCTTGAGCAGCAGCAGGTACT |
Internal bar code: | TGTCAACGGTTCGGTTACGTCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 619 |
LEAP-Seq percent confirming: | 99.8045 |
LEAP-Seq n confirming: | 1021 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTTGCCAACTTCAGCTCCTT |
Suggested primer 2: | ACCTTCACTCCCGGTTTCTT |